mmol NHS-Fluorescein = 15 = Molar excess of NHS-Fluorescein to protein 473.4 = Molecular weight of NHS-Fluorescein 100 = Microliters of solvent in which the 1mg of NHS-Fluorescein is dissolved . After functioning with the mitochondrial-targeted triphenylphosphonium (TPP) group, the . Some of the advantageous features of fluorescein fluorophores are good water solubility and absorption, emission, and maximum brightness at physiological pH (Anthoni et al., 1995) [2]. FLR-K48 TUBE HF has one fluorescein molecule conjugated to a single, defined location within the protein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. Molecular weight-dependent lymphatic transfer of fluorescein isothiocyanate-labeled dextrans after intrapulmonary administration and effects of various absorption enhancers on the lymphatic transfer of drugs in rats. Fluorescein-5-Maleimide is a sulfhydryl . Effect of the cranberry NDM fraction on degradation of type I collagen and transferrin by P. gingivalis. Fluorophores with lifetimes around 4 nsec, such as fluorescein and the rhodamines, provide a good separation in FP between small ligands and proteins of 10 kDa and higher. EXAMPLE: For 1mL of a 1mg/mL solution of IgG (150,000 Da), 4.7L NHS-Fluorescein will be used. Molecular Weight: 332.31 Molecular Formula: C20H12O5 Additional Metadata. lyophilized. fluorescein isothiocyanate (FITC), cyanine, coumarin, and quin - Solutions of SAMSA fluorescein can be made by dissolving the powder in dimethylformamide or in buffer (pH>6); storage in aqueous so-lution is not recommended . 58.5 1.8 1.8 376 0.156, or 0.16, answer. Molecular weight: 427.363 Excitation source: 488 nm spectral line, argon-ion . No offer available . Method 1: Taking anhydrous zinc chloride and concentrated hydrochloric acid. Extinction coefficient (cm -1 M -1) 80000 1. As expected, fluorescein sodium produced the greatest cumulative permeation (637.4 42.69 g). Coefficients for fluorescein (molecular weight 322 Da) and a ten kDa dextran ended up measured in a few gels, one particular of which (termed PCS) is incredibly related to the medical Tenofovir gel centered on listed here [forty]. Hexachloro-fluorescein (HEX) is hexachlorinated version of the fluorescent dye fluorescein, and is used for labeling oligonucleotides at either the 5' or 3' end. Format. OCTOBER 1998. Fluorescein. Cy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). In solution, small molecular weight fluorophores (up to several thousand Daltons) have a rotational correlation time ranging between 50 and 100 picoseconds, which is significantly faster than the rate of emission and displaces, or randomly distributes, the emission transition . Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. 50 g. Shop now or request a quote Shop Fluorescein Sodium Salt, indicator, 98.5 to 100.5%, Honeywell Fluka . Fluorescein (resorcinolphthalein) is a synthetic organic photoactive dye compound, first synthesized by von Bayer in 1871 [1]. For more information about the substance, you may click one of the links below to take you to the relevant section: Program and regulatory information about this substance, including links to EPA applications/systems, statues/regulations, or other sources . DOWNLOAD PRODUCT BROCHURE. It is frequently utilized as a luminous tracer in a wide range of applications. Excitation (nm) 498 . 23.4 kDa: Physical State. FAM can be attached to 5' or 3' end of oligos. Fluorescein alkynylamino-ATP. Some documentation and label information may refer to the legacy brand. Fluorescein A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium, such as the aqueous humor, and is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma. molecular weight (<450 daltons). Fluorescein isothiocyanate ( FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. Concentration. Color: Red: CAS: 518-47-8: Molecular Formula: C 20 H 10 Na 2 O 5: UN Number: NONH for all modes of transport . The most promising SOD9 shows rapid renal excretion and blood-brain barrier passing properties. National Institutes of Health. Info. The capacity of P. gingivalis to degrade type I collagen and transferrin was affected by NDM from cranberry ().NDM at a concentration of 50 g/mL significantly reduced the collagenase activity of P. gingivalis by 30% (Figure 3a). The high water solubility, biocompatibility and range of functionalities make fluorescent dextrans ideal for many applications. Cy3 has been used with fluorescein for double labeling; however, the use of a narrow band-pass emission . US EN. SKU: UM-0504F-0050. Shelf life of this compound is not tested, but the compound is fairly stable at RT. Calculate the sodium chloride equivalent for glycerin, a nonelectrolyte with a molecular weight of 92.2 Glycerin,ifactor 1.0 58.5 1.8 1.0 92 0.35, answer. Fluorescein. Fluorescein. 1. Fluorescent dextrans from Fina Biosolutions use our well-characterized amino dextrans as their base. Quantity. Get All The Latest News Help & Support Center English English; Change Country; 0. It is a yellowish-red compound, which fluoresces a brilliant yellow-green under ultraviolet or blue illumination (Fig. On the other hand, binding of the Evans Blue to albumin confers properties of a protein tracer. Molecular Weight: 498.4: Chemical Formula: C 27 H 18 N 2 O 8: Maximum Emission: 520 nm: Maximum Excitation: 492 nm: Required . Owing to its small molecular weight, sodium fluorescein may cross the BBB much more readily than larger molecular weight tracers. Shop Thermo Scientific Fluorescein at Fishersci.co.uk. Dimensions. . Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water). For example, the well-studied probe fluorescein isothiocyanate . 3326-32-7: Molecular Formula: C 21 H 11 NO 5 S: Molecular Weight: 389.38: Risk # 42/43: Safety # 22-36/37-45 : Technical Data : MSDS: Add to Enquiry: Add to Favourites: Classification: Dyes, Indicators and Stains COA/MSDS Molecular Weight (mw) Extinction Coeficient (ec) Technical Info (pdf) Absorbance MAX . Molecular Weight. 1995;3(4):263-71. low molecular weight (MW) protein-based macromolecules, such as recombinant antibody fragments, typically within the range of 10-70 kDa, have been . Sites of blood-brain barrier leakage were analyzed by fluorescence microscopy. Thermo Scientific Fluorescein $29.78 - $800.57 Chemical Identifiers View More Specs Products 4 Description Specifications Safety and Handling Description This Thermo Scientific brand product was originally part of the Acros Organics product portfolio. The compound should be stored at -20 C and away from . Because papaverine hydrochloride is a 2-ion electrolyte, dissociating 80%, itsifactor is 1.8. Concentration. Three milliliters of the RBITC-BSA complex was injected in healthy male Japanese rabbits weighing 2.5 kg. Description SDS Pricing; 1277037: United States Pharmacopeia (USP) Reference Standard: Expand. . ), but the non-lens wearers had longer TBUT times (14.6 and 14.5 s for the two dyes) compared with contact lens wearers (9.8 and 10.7 s, p < 0.01). Molecular Weight. The NHS-ester of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Properties of NHS-Fluorescein: Alternative name: 5/6-FAM SE Chemical name: 5/6-carboxyfluorescein succinimidyl ester Molecular weight: 473.4 Excitation source: 488 nm spectral line, argon-ion laser The mean apparent permeability P values for sodium fluorescein (molecular weight (MW) 376 Da), dextran-4k, -20k, -40k, -70k, and IgG (MW 160 kDa) were 14.6, 6.2, 1.8, 1.4, 1.3, and 0.54 10 7 cm/s, respectively. ), but the nonlens wearers had longer TBUT times (14.6 . 0.00010mmol NHS-Fluorescein mmol IgG Fluorescein derivatives are often generated through modification at the C-5 or C-6 position on the lower aromatic ring. Packaging: Box of 100 individually packed sterile strips. Fluorescein sodium has a molecular weight of 376.67. . 1000-1350 nm). Quantity. Calculators Common stock solution preparation Table 1. Note that volume is only for preparing stock solution. 1mg, 5mg, 10mg. You can purchase Streptavidin-PE and Streptavidin-APC from Molecular Probes (Cat # S-866 and S-868, respectively). Liquid. Our results failed to reveal significant differences, since the regions of interest were characterized by a similar . In cases where lens fluting is suspected and/or the patient reports discomfort with silicone hydrogel lenses at trial fitting, the lens (es) may be removed and fluorescein or high molecular weight fluorescein instilled onto the back surface of the lens before re-insertion and further evaluation of the lens fit. Applications Products Services Support. [0004] Haptens such as biotin and fluorescein are often used to conjugate with antibodies or other small drug molecules in assay reagents. 2-8C Looking for similar products? Technical Specifications. Size. The antigen tentatively termed Ku, after the first two letters of patient's name, has distinct physicochemical properties and immunological speci-ficities that distinguish it from previously reported antigens. 408.49; All; Links to Resources; Names & Synonyms; Registry Numbers; Fluorescein (molecular weight is about 332 Da) typically contains several combined aromatic groups or planar or cyclic molecules with several bonds. They come in an array of molecular weights, fluorescent dyes, dye ratios and additional functional groups. Storage-80C, avoid freeze/thaw cycles. HEX has an absorbance maximum of 535 nm and an emission maximum of 556 nm. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Results: Overall, the mean TBUT times were 12.2 5.9 s for fluorescein and 12.6 5.6 s for fluorexon (p = n.s. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. patient with connective tissue disease have been used to define a high molecular weight acidic nuclear protein antigen. Correction Factor (280 nm) 0.275. Molecular Formula: C 20 H 10 Na 2 O 5: MDL Number: . Fluorescein. Additional information. English English; Change Country. fluorescein. This solid should be stored desiccated at -20C and protected from light. Structure Search. 6-carboxyfluorescein (6-FAM) is the most commonly used fluorescent dye for labeling oligonucleotides. Heat the flask on an oil bath to 180 (internal temperature). Fluorene-based analogues of fluorescein, rhodol, and rhodamine exhibit absorption and fluorescence beyond 800-900 nm in water, 300-400 nm red-shifted compared to the original oxygen-bridged xanthene dyes, giving potential access to low molecular weight fluorescent markers for the second biological window (NIR-II, ca. Download scientific diagram | Molecular structure of fluorescein. Visit Product Comparison Guide Description Application FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. Composition per strip: Each strip is impregnated with 0.5 mg of High molecular Fluorescein Sodium. 10.2 kDa: Physical State. Molecular Weight 668.39 g/mol Concentration <0.01% SECTION 3: HAZARDS IDENTIFICATION CLASSIFICATION of the substance or mixture Not a hazardous substance or mixture. 28803 Fluorescein sodium salt (Honeywell Fluka) CAS Number: 518-47-8 . Different histological preparation methods were used. Protocols for intestinal permeability measurements in mice using 4-kDa fluorescein isothiocyanate-conjugated (FITC) dextran differ considerably among laboratories on the blood-sampling time. These measurements also confirmed the accuracy of the computed diffusion coefficient from Stokes Einstein concept. Grind 15 g of phthalic anhydride and 22 g. of resorcinol together and transfer the mixture to a conical flask. Use of a high molecular weight fluorescein (fluorexon) ophthalmic strip in assessments of tear film breakup time in contact lens wearers and noncontact lens wearers . Weight. Size. These P values were not significantly different from those of rat pial microvessels for the same-sized solutes (Yuan et al . Both of these compounds show fluorescence and are hydrophilic in nature; the excitation wavelength is between 490 and 520 nm. Additional information. Their solubilities decrease as the molecular weight increases; thus the maximum solubility in aqueous buffer is about 100 mg/mL for 3 000 MW dextrans, 50 mg/mL for 10 000 MW dextrans, 25 mg/mL for 70 000 MW . It is also suggested that changes in BBB permeability to sodium fluorescein may be the earliest and the most sensitive indicator of BBB disruption [42,43,44,45,46]. 6-FAM is reactive, water-soluble, and has an absorbance maximum of 492 nm and an emission maximum of 517 nm. Tag. At a high concentration of NDM (100 g/mL), the type I . Fluorescein. (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is . Definitions of molecular mass, molecular weight, molar mass and molar weight: Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). 6417-85-2 - DZSPMLRDMGJVAT-UHFFFAOYSA-L - Fluorescein dipotassium - Similar structures search, synonyms, formulas, resource links, and other chemical information. It has . Thermo Scientific Fluorescein-5-Maleimide is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Liquid. Purity. . National Library of Medicine. Molecular Weight. Variable. 6-FAM plays a particularly important role in real-time PCR applications, being used as a reporter moiety in TaqMan probes (1), Scorpion . National Library of Medicine. Alternative Names. Articles of Fluorescein are included as well. For evaluating contact lens fitting.
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